mouse recombinant 5 gdf9  (R&D Systems)

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Novel anti-Mullerian hormone receptor 2 binding peptide prevents chemotherapy-related ovarian follicle loss in a mouse model

doi: 10.1007/s10815-026-03902-5

Figure Lengend Snippet: Real-time RT-PCR oligonucleotide primer sequences and cycling conditions

Article Snippet: We measured colorimetric ELISA for FSH-R (Bioassay technology laboratory), Ki67 (MYBioSource), BMP15 (ABclonal Technology), GDF9 (Elabscience), SMAD 5 and SMAD 8/9 (MYBioSource; SMAD 5, MBS9714237, and SMAD 8/9, MBS9714235), following the manufacturer protocol.

Techniques: Quantitative RT-PCR, Amplification

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Novel anti-Mullerian hormone receptor 2 binding peptide prevents chemotherapy-related ovarian follicle loss in a mouse model

doi: 10.1007/s10815-026-03902-5

Figure Lengend Snippet: The effect of AMHR2BP on ovarian cortex. Follicle measurements in C57BL/6 J female mice at baseline (12 weeks old, white), or after treatment with AMHR2BP (blue), rAMH (green), or placebo (PBS, black) for 3 weeks. A Primordial follicles (PDF). B Primary follicles (PRF). C Secondary follicles (SEF). D Tertiary follicles (TRF). E Corpora lutea. Log2 fold change of real-time RT-PCR ovarian cortex tissue expression of F inhibin B, as well as G BMP15, Caspase 3 (Cas-3), FSH-R2, GDF9, and Ki67, in the AMHR2BP, rAMH, and placebo (PBS) groups compared to the baseline group. H Log2 fold change of ovarian tissue protein concentrations of BMP15, Caspase 3, FSH-R2, GDF9, and Ki67 in C57BL/6 J female mice at baseline (12 weeks old), or after treatment with AMHR2BP, rAMH, or placebo (PBS) for 3 weeks. Both treatment regimens exhibited a significant decrease in concentration of all parameters, showing inhibition of cellular function. The asterisks indicate significance of the comparisons between the different treatments using Kruskal–Wallis test for comparison of medians (* p < 0.05; ** p < 0.005)

Article Snippet: We measured colorimetric ELISA for FSH-R (Bioassay technology laboratory), Ki67 (MYBioSource), BMP15 (ABclonal Technology), GDF9 (Elabscience), SMAD 5 and SMAD 8/9 (MYBioSource; SMAD 5, MBS9714237, and SMAD 8/9, MBS9714235), following the manufacturer protocol.

Techniques: Quantitative RT-PCR, Expressing, Concentration Assay, Inhibition, Cell Function Assay, Comparison

Journal: Scientific Reports

Article Title: Changes in ovarian hardness and elasticity affect the development and function of secondary follicles

doi: 10.1038/s41598-026-39396-5

Figure Lengend Snippet: Effects of oocyte-secreted factors on secondary follicles cultured in 0.1 % alginate bead with viscosity 4-12 cP. A; The effect of oocyte-secreted factors on the development of secondary follicles with multilayered granulosa cells. Ten secondary follicles with multilayered granulosa cells were placed in each alginate bead and then were cultured for 7 days in culture medium containing 10 ng/ml ovine FSH, GDF9 (0, 10 and 100 ng/ml) and ITS Supplement in the presence of 1 % (v/v) FBS. The diameter of each secondary follicle was measured 7 days after culture. In the box plot: bar, × = median, box = 25 th to 75 th percentiles, whiskers = 10 th and 90 th percentile. N=3 animals for each treatment. The experiment was repeated three times. The different symbols represent significant differences (p < 0.05). *; Significant differences observed between days 0 and 7 in the 10 and 100 ng/mL GDF9 groups (p < 0.05). B; The effect of oocyte-secreted factors on the expression of marker genes about the follicular development, luteinization and inflammation/anti-inflammatory in granulosa cells of cultured secondary follicles. Ten secondary follicles with multilayered granulosa cells were placed in each alginate bead and then were cultured for 7 days in culture medium containing 10 ng/ml ovine FSH, GDF9 (0, 10 and 100 ng/ml) and ITS Supplement in the presence of 1 % (v/v) FBS. The diameter of each secondary follicle was measured 7 days after culture. Levels of mRNA were normalized to that of L19 . The values are the mean ± SEM. N=3 animals for each treatment. The experiment was repeated three times. The value of 0 ng/ml GDF9 was set as 1, and the data are presented as fold induction. *Significant differences observed between the 0 ng/mL and 10 or 100 ng/mL GDF9 groups (p < 0.05).

Article Snippet: Ten of secondary follicles were placed in one alginate bead and were cultured for 7 days in the medium (DMEM/F12 containing penicillin and streptomycin) containing 10 ng/ml ovine FSH (NIDDK, Torrance, CA, USA) and ITS Supplement (Sigma) in the presence of 1 % (v/v) FBS with or without mouse recombinant GDF9 (0, 10 and 100 ng/ml) (R&D Systems Inc., Minneapolis, MN) or Cytochalasin (0, 1 and 10 μM) (Sigma) at 37 °C and 5% CO 2 condition.

Techniques: Cell Culture, Viscosity, Expressing, Marker

Journal: Science Advances

Article Title: Repair of female reproductive function by GDF-9–overexpressing extracellular vesicles via ACVR1B/SMAD2 regulation in ovarian granulosa

doi: 10.1126/sciadv.adw9006

Figure Lengend Snippet: ( A ) Schematic illustration of the in vitro DO model; concentration of cisplatin, 2.7 μg/ml; EV/GOE, 1 × 10 6 particles/ml; time of treatment, 24 hours (h). ( B ) Flow cytometry analysis of GC viability, apoptosis, and cell death. ( C to E ) Quantification of flow cytometry data, showing the efficacy of EVs in treating DO in vitro. ( F ) No statistically significant difference in apoptosis rates between GOEs and GDF-9 –overexpressing MSCs. n = 3, data are presented as means ± SD. This figure was created in part using BioRender.com . Created in BioRender. Zhang, S. (2025) https://BioRender.com/zv1tbzt . IC 50 , median inhibitory concentration. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant.

Article Snippet: The protein concentration of GDF-9 in control EVs and GOE was quantitatively analyzed using the ELISA kit (Raybiotech, ELH-GDF9-1).

Techniques: In Vitro, Concentration Assay, Flow Cytometry